UV-Vis Spectroscopy

Gold Nanoparticle
Concentration

Calculates particle concentration from absorbance at 400 nm.
Supports nanorods (L > W) and nanospheres (L = W).
Optional: surface area, ligand coverage and ligand amount for exchange (MUTAB, PEG-SH 2000).
Reference: Abs 1.2 ≡ 0.5 mM Au atoms · Sample volume: 1 mL

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Input Parameters
nm
nm
a.u.
enter dimensions above
Results
—
Particle concentration
nM
Particle Volume —
Au Atoms / Particle —
Total Au Atoms (1 mL) —
Total Particles (1 mL) —
Aspect Ratio —
Au Atom Concentration —
Surface Area / Particle —
Total Au Surface (1 mL) —
Atoms per particle (scale) —
Ligand addition to 1 mL sample · 100× excess over surface sites
MUTABPEG-SH 2000
Ligands / particle——
Surface sites (1 mL)——
Ligand to add——
Mass to add——
Stock volume——

Ligands / particle = surface area × packing density. Mass = excess × surface sites in 1 mL / NA × M. Stock volume = mass / stock concentration (dilution of the sample by the added volume is neglected).